Arroyo-Pérez E*, Hook, JC, Alvarado A, Wimmi S, Glatter, T, Thormann KM*, Ringgaard S. (2023) A conserved cell-pole determinant organizes proper polar flagellum formation. bioRXiv


Luthe T, Kever L, Thormann K, Frunzke J. Bacterial multicellular behavior in antiviral defense. Curr Opin Microbiol. 2023 Apr 6;74:102314. doi: 10.1016/j.mib.2023.102314.


Kreiling V, Thormann KM (2023) Polarity of c-di-GMP synthesis and degradation. microLife 4, uqad014,


Schwan M, Khaledi A, Willger S, Papenfort K, Glatter T, Häußler S, Thormann KM (2022) FlrA-independent production of flagellar proteins is required for proper flagellation in Shewanella putrefaciens. Mol Microbiol. doi: 10.1111/mmi.14993


Kühn, M. J., Edelmann, D. B., and K. M. Thormann (2022) Polar flagellar wrapping and lateral flagella jointly contribute to Shewanella putrefaciens environmental spreading. Environ. Microbiol.


Thormann, K. M., Beta, C., and M. J. Kühn (2022) Wrapped up: The motility of polarly flagellated bacteria. Annu. Rev. Microbiol. 1: doi: 10.1146/annurev-micro-041122-101032


Rick, T., Kreiling, V., Höing, A., Fiedler, S., Glatter, T., Steinchen, W., Hochberg, G., Bähre, H., Seifert, R., Bange, G., Knauer, S. K., Graumann, P., and K. M. Thormann (2022) GGDEF domain as spatial on-switch for a phosphodiesterase by interaction with landmark protein HubP. npj Biofilms Microbiomes 8, 35.


K. M. Thormann (2022) Dynamic hybrid flagellar motors - fuel switch and more. Front. Microbiol.


Chernova, L., and K. M. Thormann (2022) Mikrokumpel. BoD, Norderstedt, ISBN 9783754337431.


Ona, L., Giri, S., Avermann, N., Kreienbaum, M., Thormann, K. M., and C. Kost (2021) Obligate cross-feeding expands the metabolic niche of bacteria. Nat. Ecol. Evol. 5: 1224-1232. (


Mayer, B., Schwan, M., Thormann, K. M., and P. L. Graumann (2021) Antibiotic Drug screening and Image Characterization Toolbox (A.D.I.C.T.): a robust imaging workflow to monitor antibiotic stress response in bacterial cells in vivo. F1000Research 10:277 (


Pecina, A., Schwan, M., Blagotinsek, V., Rick, T., Klüber, P., Leonhard, T., Bange, G., and K. M. Thormann (2021) The stand-alone PilZ-domain protein MotL specifically regulates the activity of the secondary lateral flagellar system in Shewanella putrefaciens. Front. Microbiol.12: 668892. doi: 10.3389/fmicb.2021.668892 & Corrigendum


Mayer, B., Schwan, M., Oviedo-Boganegra, L. M., Bange, G., Thormann, K. M., and P. L. Graumann (2021) Dynamics of bacterial signal recognition particle at a single molecule level. Front. Microbiol. 12: 793. DOI: 10.3389/fmicb.2021.663747


Hook, J. C., Blagotinsek, V., Pané-Farré, J., Mrusek, D., Altegoer, F., Dornes, A., Schwan, M., Schier, L., Thormann, K. M.*, and G. Bange* (2020) A proline-rich element in the type III secretion protein FlhB contributes to flagellar biogenesis in the beta- and gamma-proteobacteria. Front. Microbiol. 11: 2908. DOI: 10.3389/fmicb.2020.564161


Kreienbaum, M., Dörrich, A.K., Brandt, D., Schmid, N.E., Leonhard, T., Hager, F., Brenzinger, S., Hahn, J., Glatter, T., Ruwe, M., Briegel, A., Kalinowski, J., and K. M. Thormann (2020) Isolation and characterization of Shewanella phage Thanatos infecting and lysing Shewanella oneidensis and promoting nascent biofilm formation. Front. Microbiol. 11: 573260. doi: 10.3389/fmicb.2020.573260.


Blagotinsek, V., Schwan, M., Steinchen, W., Mrusek, D., Hook, J. C., Rossmann, F., Freibert, S. A., Kratzat, H., Murat, G., Kressler, D., Beckmann, R., Beeby, M., Thormann, K. M.*, and G. Bange* (2020) An ATP-dependent partner switch links flagellar C-ring assembly with gene expression. Proc. Natl. Acad. Sci. U. S. A. 117(34): 20826-20835. doi:10.1073/pnas.2006470117


Kühn, M., and K. M. Thormann (2020) Eine Flagelle für alle Fälle. Biospektrum 3.20


Nisbett, L. M., Binnenkade, L., Bacon, B. A., Hossain, S., Kotloski, N. J., Brutinel, E. D., Hartmann, R., Drescher, K., Arora, D. P., Muralidharan, S., Thormann, K., Gralnick, J. A., and E. M. Boon (2019) NosP signaling modulates the NO/H-NOX-mediated multicomponent c-di-GMP network and biofilm formation in Shewanella oneidensis. Biochemistry. doi: 10.1021/acs.biochem.9b00706.


Díaz-Pascual, F., Hartmann, R., Lempp, M., Vidakovic, L., Song, B., Jeckel, H., Thormann, K. M, Yildiz, F. H., Dunkel, J., Link, H., Nadell, C. D., and K. Drescher (2019) Breakdown of Vibrio cholerae biofilm architecture induced by antibiotics disrupts community barrier function. Nat. Microbiol. doi:10.1038/s41564-019-0579-2


Ferreira, J. L., Gao, F. Z., Rossmann, F. M., Nans, A., Brenzinger, S., Hosseini, R., Wilson, A., Briegel, A., Thormann, K. M., Rosenthal, P. B., and M. Beeby (2019) γ-proteobacteria eject their polar flagella under nutrient depletion, retaining flagellar motor relic structures. PLoS Biology


Lerch, M. F., Schoenfelder S. M. K., Marincola, G., Wencker, F. D. R., Eckart, M., Förstner, K. U., Sharma C. M., Thormann, K. M., Kucklick, M., Engelmann, S., and W. Ziebuhr (2019). A non-coding RNA from the intercellular adhesin (ica) locus of Staphylococcus epidermidis controls polysaccharide intercellular adhesin (PIA)-mediated biofilm formation. Mol. Microbiol. doi: 10.1111/mmi.14238.


Rossmann, F. M., Rick, T., Mrusek, D., Sprankel, L., Dörrich, A. K., Leonhard, T., Bubendorfer, S., Kaever, V., Bange, G., and K. M. Thormann (2019) The GGDEF domain of the phosphodiesterase PdeB in Shewanella putrefaciens mediates recruitment by the polar landmark protein HubP. J. Bacteriol. DOI: 10.1128/JB.00534-18


Kühn, M. J., Schmidt, F. K., Farthing, N. E., Rossmann, F. M., Helm, B., Wilson, L. G., Eckhardt, B., and K. M. Thormann (2018) Spatial arrangement of several flagellins within bacterial flagella improves motility in different environments. Nat. Commun. 9: 5369


Binnenkade, L., Kreienbaum, M., and K. M. Thormann (2018) Characterization of ExeM, an extracellular nuclease of Shewanella oneidensis MR-1. Front. Microbiol. 9: 1761


Brenzinger, S., Pecina, A., Mrusek, D., Mann, P., Völse, K., Wimmi, S., Ruppert, U., Becker, A., Ringgaard, S., Bange, G., and K. M. Thormann (2018) ZomB is essential for flagellar motor reversals in Shewanella putrefaciens and Vibrio parahaemolyticus. Mol. Microbiol. 109: 694-709


Hintsche, M., Waljor, V., Großmann, R., Kühn, M. J., Thormann, K. M., Peruani, F., and C. Beta (2017) A polar bundle of flagella can drive bacterial swimming by pushing, pulling, or coiling around the cell body. Scientific Reports 7:16771


Kühn, M. J., Schmidt, F. K., Eckhardt, B., and K. M. Thormann (2017) Bacteria employ a polymorphic instability of the flagellar filament to escape from traps. Proc. Natl. Acad. Sci. U S A


Brenzinger, S., and K. M. Thormann (2017) Dynamics in the dual fuel flagellar motor of Shewanella oneidensis MR-1. Methods Mol. Biol. 1593: 285-295.


Brenzinger, S., Dewenter, L., Delalez, N. J., Leicht, O., Berndt, V., Paulick, A., Berry, R. M., Thanbichler, M., Armitage, J. P., Maier, B., and K. M. Thormann (2016) Mutations targeting the plug-domain of the Shewanella oneidensis proton-driven stator allow swimming at increased viscosity and under anaerobic conditions. Mol. Microbiol. 102: 925-938


Dwarakanath, S., Brenzinger, S., Gleditzsch, D., Plagens, A., Klingl, A., Thormann, K., and L. Randau (2015) Interference activity of a minimal Type I CRISPR-Cas system from Shewanella putrefaciens. Nucleic Acids Res. 43: 8913-8923


Schuhmacher, J. S., Thormann K. M., and G. Bange (2015) How bacteria maintain location and number of flagella? FEMS Microbiol. Rev. 39: 812-822


Rossmann, F., Brenzinger, S., Knauer, C., Dörrich, A. K., Bubendorfer, S., Ruppert, U., Bange, G., and K. M. Thormann (2015) The role of FlhF and HubP as polar landmark proteins in Shewanella putrefaciens CN-32. Mol. Microbiol. 98: 727-742


Schuhmacher, J. S., Rossmann, F., Dempwolff, F., Knauer, C., Altegoer, F., Steinchen, W., Dörrich, A. K., Klingl, A., Stephan, M., Linne, U., Thormann, K. M., and G. Bange (2015) MinD-like ATPase FlhG effects location and number of bacterial flagella during C-ring assembly. Proc. Natl. Acad. Sci. U S A 112: 3092-3097


Paulick, A., Delalez, N. J., Brenzinger, S., Steel, B. C., Berry, R.M., Armitage, J. P., and K. M. Thormann (2015) Dual stator dynamics in the Shewanella oneidensis MR-1 flagellar motor. Mol. Microbiol. 96: 993-1001


Nanda, A. M., Thormann, K., and J. Frunzke (2014) Impact of spontaneous prophage induction on the fitness of bacterial populations and host-microbe interactions. J. Bacteriol. 197: 410-419


Bubendorfer, S., Koltai, M., Rossmann, F., Sourjik, V., and K. M. Thormann (2014) Secondary bacterial flagellar system improves bacterial spreading by increasing the directional persistence of swimming. Proc. Natl. Acad. Sci. U S A 111: 11485-11490


Binnenkade, L., Teichmann, L., and K. M. Thormann (2014) Iron triggers λSo prophage induction and release of extracellular DNA in Shewanella oneidensis MR-1 biofilms. Appl. Environ. Microbiol. 80: 5304-5316


Zweig, M., Schork, S., Koerdt, A., Siewering, K., Sternberg, C., Thormann, K., Albers, S.V., Molin, S., and C. van der Does (2014) Secreted single-stranded DNA is involved in the initial phase of biofilm formation by Neisseria gonorrhoeae. Environ. Microbiol. 16: 1040-1052


Bubendorfer, S., Ishihara, M., Dohlich, K., Heiss, C., Vogel, J., Sastre, F., Panico, M., Hitchen, P., Dell, A., Azadi, P, and K. M. Thormann. 2013. Analyzing the modification of the Shewanella oneidensis MR-1 filament. PLoS ONE 8:e73444


Lassak, J., Bubendorfer, S., and K. M. Thormann. 2013. Domain analysis of ArcS, the hybrid sensor kinase of the Shewanella oneidensis MR-1 Arc two-component system, reveals functional differentiation of its two receiver domains. J. Bacteriol. 195: 482-492


Gödeke, J., Binnenkade, L., and K. M. Thormann. 2012. Transcriptome analysis of early surface-associated growth of Shewanella oneidensis MR-1. PLoS One 5: e42160


Paulick, A., and K. M. Thormann. 2012. Bakterielles Motortuning. BIOspektrum 18: 134-137


Heun, M., Binnenkade, L., Kreienbaum, M., and K. M. Thormann. 2012. Functional specificity of extracellular nucleases in Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 78: 4400-4011


Bubendorfer, S., Held, S., Windel, N., Paulick, A., Klingl, A., and K. M. Thormann. 2012. Specificity of motor components in the dual flagellar system of Shewanella putrefaciens CN-32. Mol. Microbiol. 83: 335-350


Gödeke, J., Heun, M., Bubendorfer, S., Paul, K., and K. M. Thormann. 2011. Roles of two Shewanella oneidensis MR-1 extracellular endonucleases. Appl. Environ. Microbiol. 77: 5342-5351


Binnenkade, L., Lassak, J., and K. M. Thormann. 2011. Analysis of the BarA/UvrY two-component system in Shewanella oneidensis MR-1. PLoS One 6: e23440


Koerdt, A., Gödeke, J., Berger, J., Thormann, K. M., and S.-V. Albers. 2010. Archael biofilm formation under extreme conditions. PLoS One 5: e14104


Thormann, K. M., and A. Paulick. 2010. Tuning the flagellar motor. Microbiology 156: 1275-1283


Gödeke, J., Paul, K., Lassak, J., and K. M. Thormann. 2010. Phage-induced lysis enhances biofilm formation in Shewanella oneidensis MR-1. ISME J. 5: 613-626


Lassak, J., Henche, A.-L., Binnenkade, L., and K. M. Thormann. 2010. ArcS, the cognate sensor kinase in an atypical Arc system of Shewanella oneidensis MR-1. Appl. Environ. Microbiol. 76: 3263-3274


Koerdt, A., Paulick, A., Mock, M., Jost, K., and K. M. Thormann. 2009. MotX and MotY are required for flagellar rotation in Shewanella oneidensis MR-1. J. Bacteriol. 191: 5085-5093


Klüsener, S., Aktas, M., Thormann, K. M., Wessel, M., and F. Narberhaus. 2009. Expression and physiological relevance of Agrobacterium tumefaciens phosohatidylcholine biosynthesis genes. J. Bacteriol. 191: 365-374


Paulick, A., Koerdt, A., Lassak, J., Huntley, S., Wilms, I., Narberhaus, F., and K. M. Thormann. 2009. Two different stator systems drive a single polar flagellum in Shewanella oneidensis MR-1. Mol. Microbiol. 71: 836-850


Thormann, K. M., Saville, R. M., Duttler, S. A., Shukla, S., and A. M. Spormann. 2006. Control of formation and cellular detachment from Shewanella oneidensis MR-1 biofilms by cyclic-di-GMP. J. Bacteriol. 188: 2681-2691


Thormann, K. M., Saville, R. M., Shukla, S., and A. M. Spormann. 2005. Induction of rapid detachment in Shewanella oneidensis MR-1 biofilms. J. Bacteriol. 187: 1014-1021


Schaffer, S., K. Thormann, S. Hujer, and P. Dürre. 2005. Quantitative proteome analysis of clostridia. In: P. Dürre and H. Bahl (eds.), Handbook on Clostridia. CRC Press, Boca Raton, FL.


Spormann, A. M., K. Thormann, R. Saville, S. Shukla, and P. Entcheva. 2004. Microbial biofilms. In: R. S. Greco, F. B. Prinz, and R. L. Smith (eds.), Nanotechnology. CRC Press, Boca Raton, FL.


Thormann, K. M., Saville, R. M., Shukla, S., Pelletier, D. A., and A. M. Spormann. 2004. Initial phases of biofilm formation in Shewanella oneidensis MR-1. J. Bacteriol. 186: 8096-8104.


Zickner, B., M. Böhringer, S. Nakotte, S. Schaffer, K. Thormann, and P. Dürre. 2003. Multiple levels of regulation of solventogenesis in Clostridium acetobutylicum.  In: P. Dürre and B. Friedrich (eds.), Regulatory networks in prokaryotes. Horizon Scientific Press, Norfolk, UK.


Dürre, P., Böhringer, M., Nakotte, S., Schaffer, S., Thormann, K., and B. Zickner. 2002. Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. J. Mol. Microbiol. Biotechnol. 4: 295-300


Thormann, K., Feustel, L., Lorenz, K., Nakotte, S., and P. Dürre. 2002. Control of butanol formation in Clostridium acetobutylicum by transcriptional activation. J. Bacteriol. 184: 1966-73


Thormann, K., and P. Dürre. 2001. Orf5/SolR: a transcriptional repressor for the sol operon of Clostridium acetobutylicum? J. Ind. Microbiol. Biotechnol. 27: 307-313


AG Thormann

Department for Microbiology and Molecular Biology

Justus-Liebig-Universität Gießen

Heinrich-Buff-Ring 26-32 | 35392 Gießen | Germany

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the dual flagellar system of Shewanella putrefaciens